A Subtractive Proteomics Approach to Identify Putative Drug Targets Against Parasitic Species

Introduction: Parasitic diseases affecting humans continue to be the leading cause of morbidity as well mortality, particularly in tropical and subtropical countries. With rise multi-drug resistant forms many diseases, it has become increasingly important develop new strategies identify alternative drug targets. One such strategy for analysis protein sequences pathogen that can provide useful insight markers target identification is Subtractive Proteomics Approach (SPA). In this dataset between host proteome subtracted analysed which provides information pertaining a set proteins are likely essential but absent host. The subtractive (SPA) one most efficient methods includes use various precise software databases. Aim: SPA was predict putative targets parasitic species Leishmania major, Plasmodium falciparum, Trypanosoma brucei cruzi. Methods: Common these were predicted using Transporter Database 2.0. analyzed by BlastP tool non-homologous homo sapiens. Essential Genes (DEG) database used parasites results subjected CELLO subcellular localization Prosite identified families patterns present Results: Drug cruzi mitochondrial Carrier family, Major Facilitator Superfamily, Sulfate Permease Family, P-type ATPase Superfamily Intrinsic Protein Family respectively. Conclusion: Screening approach major not human investigated further computational designing systems biology approach.